Research Electron Microscopy Service

The Duke Electron Microscopy Service is a resource for high quality light and electron micrographs and is the longest continuously running shared service facility on campus with a history of almost four decades.

This facility provides a full range of sample preparation, technical assistance, and training in viewing and photographing specimens. We supply ultra-structural analyses of small specimens, including cells and tissues, sub-cellular organelles, and microorganisms, such as viruses, bacteria, fungi, and parasites. We can provide data, train users, and assist in design of experimental procedures. For new experiments by inexperienced users, it is recommended that you consult EM staff before a project is started to ensure proper handling. There is no charge for consults.

For information on use of the facility, capabilities, or specimen preparation, contact:

Capabilities

• Transmission Electron Microscopy
• High resolution digital imaging (2k x 2k camera)
• Ultra-microtomy (60-90 nm thin sections of cells and tissues)
• Ultra-cryomicrotomy (thin sections of cells and tissues for immune-labeling)
• Negative staining of viruses and sub-cellular organelles
• Immuno-labeling of particulate specimens
• “Vibratome” (vibrating tissue slicer sectioning) sectioning
• Pre-embedding immune-labeling
• Silver-enhanced small tag immune-labeling
• Microwave specimen processing
• High-resolution (12 mp camera) photomicroscopy (dissecting and conventional light microscope)
• Stereoscopy with darkfield
• Digital image processing; stitching, extended focus, measurement
• Scanning Electron Microscopy (SEM)
• Critical point drying for SEM
• Metal coating for SEM
• Microprobe elemental analysis