The Research Histology Laboratory is an integral and essential component of the Department of Pathology. It functions as the histology laboratory of the BioRepository & Precision Pathology Center, with the primary role of providing specialized support to the autopsy service and multiple investigators at Duke. The Research Histology Laboratory is a state-of-the-art facility that provides routine and specialized histological services, as well as immunohistochemical stains. The staff provides assistance with respect to the proper collection and fixation of the tissue, immunohistochemical staining procedures, and the evaluation of the stains.

Hours of Operation: Monday-Friday, 8am-6pm
Designated drop off and pick up times: 9:30-11:00am; 3:00-4:30pm
Location for General Histology Services: Duke South Green Zone, Room 254

Contact Information
Phone: 919-684-6209
Email

Director: Shannon J. McCall MD,   919-684-2531
Research Program Leader:
Laboratory Manager:
Scientific Director: Zuowei Su PhD,  919-684-1727

Staff:
Emily Knutson, HT (ASCP)
Ronald Patterson, HT
Laura Sparks, HT
Jeffrey Groth, HT (ASCP); part time

Services Offered

• Routine paraffin processing, embedding, and sectioning
• Hematoxylin and eosin (H+E)
• Frozen Sectioning
• Immunohistochemical Staining
• Special staining
• Tissue microarray creation

Stains

Please call if you need a stain not listed here. Contact Dr. Zuowei Su at  919-684-1727 to discuss your immunostaining needs, and see the list of our currently available stains. If the antibody you need is not on our list, then please contact Dr. Su for advice and assistance. A list of some commonly requested stains are:

• PHF-TAU
• Beta Amyloid Protein (BAPP)
• Alpha-Synuclein
• BAF47, CD3, CD4, CD8, CD15, CD31, CD34, CK(AE1/AE3), CK14
• Cleaved Caspase 3
• Estrogen Receptor Alpha, F4/80, FoxP3, GRP78, HAM56, HMB45, Ki67, GFAP, Mart-1, Neurofilament (NFP), p53, S100, Synaptophysin

Special Stains

• Congo Red
• Cresyl Violet
• Luxol Fast Blue
• Mikat
• Masson Trichrome
• Martius Scarlett Blue (MSB)
• Oil Red O (frozen sections only)
• Periodic Acid Schiff (PAS)
• Periodic Acid Schiff – Diastase Digestion (PAS-D)
• Toluidine Blue

Instrumentation

•  Sakura Tissue Tek VIP 5 tissue processors
• Sakura Tissue Tek 5 tissue embedding console
• Leica RM2235 manual microtome
• Thermo Scientific Microm HM355S automated microtomes
•  Leica CM 1800 cryostats
• Thermo Scientific CryoStar NX70 cryostat
•  Leica Autostainer XL H&E Stainer
• Medite RCM7000 automatic coverslipper
• Ventana Discovery Ultra automated immunohistochemistry staining system
• Caliper Life Sciences TMA Master tissue microarrayer